abstract：:We describe here the presence of ubiquitin and its conjugation system in the rat retina. Retinal homogenates and supernatants conjugate [125I]human ubiquitin with either endogenous or exogenous proteins. The conjugating activity is relatively stable over time, requires ATP, and has a pH optimum of approximately 8. The...

journal_title：Journal of neuroscience research

authors： Naash M,Izbicka E,Anderson RE

更新日期：1991-10-01 00:00:00

abstract：:The growth cone, the motile tip of developing neuronal processes, is considered responsible for the exact guidance of axons and synaptogenesis. High activity of tyrosine kinases in growth cones may contribute to the functions of growth cones. Our previous work revealed that vinculin is one of the endogenous substrates...

journal_title：Journal of neuroscience research

authors： Igarashi M,Komiya Y

更新日期：1991-09-01 00:00:00

abstract：:Tau microtubule-associated proteins are believed to play a role in regulation of the growth of neuronal processes. In order to study the function of tau protein in vivo, we examined the inhibition of tau expression in PC12 cells by exposing the cells to tau antisense oligodeoxynucleotides. A specific retraction of neu...

journal_title：Journal of neuroscience research

authors： Hanemaaijer R,Ginzburg I

更新日期：1991-09-01 00:00:00

abstract：:Double immunofluorescence studies using antibodies against NF-L and peripherin revealed three distinct subpopulations of neurons in rat dorsal root ganglia (DRG). In the adult rat, 46% of the DRG neurons were small and peripherin-positive (NF-L-negative), and 48% were large and NF-L-positive (peripherin-negative). Abo...

journal_title：Journal of neuroscience research

authors： Goldstein ME,House SB,Gainer H

更新日期：1991-09-01 00:00:00

abstract：:Fibroblast growth factors (FGFs) are known to be synthesized in the central nervous system (CNS) and to act on CNS cells in vitro, but less is known about their synthesis, expression, and role in vivo. In this work, using specific anti-acidic fibroblast growth factor (aFGF) antibodies, we have shown for the first time...

journal_title：Journal of neuroscience research

authors： Tourbah A,Oliver L,Jeanny JC,Gumpel M

更新日期：1991-08-01 00:00:00

abstract：:Previous studies from our laboratory suggest that Alzheimer's disease sera contain a repertoire of antibodies to the heavy neurofilament subunit (NF-H) and that a subpopulation of these antibodies bind specifically to epitopes highly enriched in NF-H isolated from the purely cholinergic electromotor neurons of Torpedo...

journal_title：Journal of neuroscience research

authors： Faigon M,Hadas E,Alroy G,Chapman J,Auerbach JM,Michaelson DM

更新日期：1991-08-01 00:00:00

abstract：:To determine whether one or more opioid receptor types might be preferentially involved in gliogenesis, primary mixed glial cultures derived from mouse cerebra were continuously treated with varying concentrations of opioid agonists selective for mu (mu), i.e., DAGO ([D-Ala2, MePhe4, Gly(ol)5]enkephalin), delta (delta...

journal_title：Journal of neuroscience research

authors： Stiene-Martin A,Hauser KF

更新日期：1991-08-01 00:00:00

abstract：:Interleukin-1 receptor antagonist (IL-1ra), a true antagonist of the interleukin-1 (IL-1) receptors, is released by activated macrophages and binds specifically to the IL-1 receptors without triggering IL-1 effects. Following peripheral nerve axotomy, activated macrophages release IL-1, which induces the expression of...

journal_title：Journal of neuroscience research

authors： Guénard V,Dinarello CA,Weston PJ,Aebischer P

更新日期：1991-07-01 00:00:00

abstract：:Calcium-activated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The muM and mM Ca(2+)-sensitive (mu- and mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the latter step enabling removal of ...

journal_title：Journal of neuroscience research

authors： Banik NL,DeVries GH,Neuberger T,Russell T,Chakrabarti AK,Hogan EL

更新日期：1991-07-01 00:00:00

abstract：:Primary fibroblasts initiated from skin biopsies of Wistar rats were transfected with a plasmid that encodes the human growth hormone and the neomycin resistance genes. Cell clones selected for G418 resistance and expressing high levels of human growth hormone were propagated in vitro and subsequently transplanted int...

journal_title：Journal of neuroscience research

authors： Doering LC,Chang PL

更新日期：1991-07-01 00:00:00

abstract：:The presence of acetylcholinesterase has been detected in the thymus of several species both biochemically and histochemically. In this study we have investigated the molecular forms and the level of this enzyme in separate compartments of the murine thymus and in different thymocyte subpopulations. Similar levels of ...

journal_title：Journal of neuroscience research

authors： Rossi A,Vicini E,Scarsella G,Biagioni S

更新日期：1991-06-01 00:00:00

abstract：:Amyloid plaques, found in characteristically large numbers in specific brain areas of Alzheimer's disease (AD) and Down's Syndrome (DS) patients, are composed of a 41-43 amino acid peptide, A4, derived from a transmembrane glycoprotein, amyloid precursor protein (APP). In transformed cells APP has been shown to be cle...

journal_title：Journal of neuroscience research

authors： Baskin F,Rosenberg RN,Greenberg BD

更新日期：1991-05-01 00:00:00

abstract：:A novel approach was developed to raise a panel of monoclonal antibodies (mAb) against brain antigens using Xenopus oocytes as immunological vectors. Xenopus oocytes were injected to express proteins encoded by brain-derived mRNA extracted from rat cerebral cortex. A crude membrane preparation from mRNA-injected oocyt...

journal_title：Journal of neuroscience research

authors： Matute C,Tigyi GJ,Miledi R

更新日期：1991-05-01 00:00:00

abstract：:The cell line PC12, derived from a rat pheochromocytoma, has served as a model for studies on the mechanism of action of nerve growth factor, as well as for the exploration of neuronal differentiation in general. When treated with nanomolar concentrations of nerve growth factor, these neoplastic chromaffin-like cells ...

journal_title：Journal of neuroscience research

authors： Simpson DL,Dickens G,Doll S,Koizumi S,Tocco M,Okuda O,Oshima M,Rudkin BB,Brightman M,Guroff G

更新日期：1991-04-01 00:00:00

abstract：:A low concentration of transition metal ions Co2+ and Ni2+ increases the inward current density in neurons from the land snail Helix aspersa. The currents were measured using a single electrode voltage-clamp/internal perfusion method under conditions in which the external Na+ was replaced by Tris+, the predominant ext...

journal_title：Journal of neuroscience research

authors： Kim YK,Woodruff ML

更新日期：1991-04-01 00:00:00

abstract：:Only a few proteins are known to be exclusively expressed in central nervous system (CNS) myelin. A novel surface membrane protein expressed only in CNS myelin and oligodendrocytes of higher vertebrates has been identified by a monoclonal antibody. This CNS myelin/oligodendrocyte-specific protein, MOSP, has a molecula...

journal_title：Journal of neuroscience research

authors： Dyer CA,Hickey WF,Geisert EE Jr

更新日期：1991-04-01 00:00:00

abstract：:There is growing evidence on the existence of endogenous ouabain-like factors that modulate Na+, K(+)-ATPase activity. In this laboratory, two soluble subfractions (peaks I and II) were previously separated from rat cerebral cortex, which had opposite effects on Na+, K(+)-ATPase activity. Peak I stimulated and peak II...

journal_title：Journal of neuroscience research

authors： Antonelli M,Casillas T,Rodríguez de Lores Arnaiz G

更新日期：1991-03-01 00:00:00

abstract：:The effects of glutamate on intracellular free Ca2+, [Ca2+]i, and neurotoxicity were compared in cerebellar granule neurons in vitro. [Ca2+]i was measured with fura-2 and digital fluorescence imaging microscopy; neurotoxicity was monitored using a vital dye and colorimetric analysis. Glutamate produced dose-dependent ...

journal_title：Journal of neuroscience research

authors： Milani D,Guidolin D,Facci L,Pozzan T,Buso M,Leon A,Skaper SD

更新日期：1991-03-01 00:00:00

abstract：:Using a solution-hybridization assay and specific oligonucleotidic probes, we have studied IGF-I and insulin receptor mRNAs in the rat central nervous system during development. The expression of mRNAs was maximal at embryonic day 15 and 20 for IGF-I receptors, and at embryonic day 20 and the day of birth for insulin ...

journal_title：Journal of neuroscience research

authors： Baron-Van Evercooren A,Olichon-Berthe C,Kowalski A,Visciano G,Van Obberghen E

更新日期：1991-02-01 00:00:00

abstract：:Several recently discovered lines of evidence support the involvement of myelin basic protein (BP)-specific T cells in multiple sclerosis (MS). To identify potentially relevant immunodominant T cell epitopes, human BP (Hu-BP)-reactive T cell lines were selected from MS and normal donors and tested for reactivity to cl...

journal_title：Journal of neuroscience research

authors： Chou YK,Henderikx P,Vainiene M,Whitham R,Bourdette D,Chou CH,Hashim G,Offner H,Vandenbark AA

更新日期：1991-02-01 00:00:00

abstract：:In cultured Schwann cells, single-channel and whole-cell K+ currents can be activated by depolarizing the membrane to values more negative than -50 mV. In elevated extracellular K+ concentration ([K+]o), however, single-channel activity and whole-cell currents could be recorded at more negative potentials. Thus, the t...

journal_title：Journal of neuroscience research

authors： Verkhratsky A,Hoppe D,Kettenmann H

更新日期：1991-02-01 00:00:00

abstract：:We have studied the interaction between several growth factors to promote parasympathetic neuronal survival. Neither insulin nor insulin-like growth factor-I (IGF-I) had any effect on the survival of embryonic day 8 chick ciliary neurons in culture. Similarly, the protein kinase C activator phorbol dibutyrate (PdBu) h...

journal_title：Journal of neuroscience research

authors： Crouch MF,Hendry IA

更新日期：1991-01-01 00:00:00

abstract：:Four new monoclonal antibodies to the extracellular domain of the nerve growth factor receptor (NGFR) have been evaluated for their specificity to NGFR and their utility in localizing NGFR in human brain. All four antibodies, as well as Me20.4, show similar cellular localization and patterns of immunoreactivity in bas...

journal_title：Journal of neuroscience research

authors： Loy R,Heyer D,Clagett-Dame M,DiStefano PS

更新日期：1990-12-01 00:00:00

abstract：:The inherited deficiency of galactosylceramide beta-galactosidase (E.C. 3.2.1.46: galactocerebrosidase) activity results in globoid cell leukodystrophy in humans (Krabbe disease) and in mice (twitcher mutant). To determine whether Krabbe patients' cells complement twitcher cells to produce, in hybrid combination, grea...

journal_title：Journal of neuroscience research

authors： Skiba MC,Lyerla TA,Konola JT,Raghavan S

更新日期：1990-12-01 00:00:00

abstract：:The iron transport protein, transferrin, and the iron storage protein ferritin were examined immunohistochemically along with iron in a number of brain regions from normal and aged humans. Two age groups were examined: a middle-aged group (28-49 years), and an older group (60-90 years). Transferrin, ferritin, and iron...

journal_title：Journal of neuroscience research

authors： Connor JR,Menzies SL,St Martin SM,Mufson EJ

更新日期：1990-12-01 00:00:00

abstract：:Laminin mediates neural adhesion and process formation. A possible signal transduction pathway for laminin was investigated in both NG108-15 and PC12 neuronal cells using radiolabeling studies as well as various stimulators and inhibitors of phosphatases and kinases. Using [32P]-ortho-phosphate, laminin caused a decre...

journal_title：Journal of neuroscience research

authors： Weeks BS,DiSalvo J,Kleinman HK

更新日期：1990-11-01 00:00:00

abstract：:Glucocorticoid hormones affect gene expression directly at the level of transcription via intracellular receptors that translocate to the nucleus in the presence of steroid. In the brain, two types of high-affinity receptors bind glucocorticoids, the type I, mineralocorticoid receptor and the type II, glucocorticoid r...

journal_title：Journal of neuroscience research

authors： Vielkind U,Walencewicz A,Levine JM,Bohn MC

更新日期：1990-11-01 00:00:00

abstract：:Dissociated embryonic rat ventral mesencephalon containing the developing A8-A10 dopamine (DA) neurons was cultured alone or in the presence of a 10 mm segment of adult rat sciatic nerve that had been explanted and maintained in separate culture for 72 hours prior to introduction to mesencephalic cultures. Nerve segme...

journal_title：Journal of neuroscience research

authors： Collier TJ,Sladek CD,Gallagher MJ,Gereau RW 4th,Springer JE

更新日期：1990-11-01 00:00:00

abstract：:Cultures of chicken day 8 embryo retinal cells, essentially free of contaminating non-neuronal elements, were used to examine the neurotoxicity of various excitatory amino acid transmitter receptor agonists. At 7 days in vitro, N-methyl-D-aspartate (NMDA), following 24 hr exposure to 0.1-1.0 mM, destroyed 60-70% of th...

journal_title：Journal of neuroscience research

authors： Facci L,Leon A,Skaper SD

更新日期：1990-10-01 00:00:00

abstract：:The expression of many myelin-specific molecules in Schwann cells is profoundly decreased following denervation. This study examines the early reexpression of myelin protein genes associated with reinnervation. Following sciatic nerve crush, the distal, regenerated nerve was divided into appropriate (2.5 or 5 mm) cons...

journal_title：Journal of neuroscience research

authors： Mitchell LS,Griffiths IR,Morrison S,Barrie JA,Kirkham D,McPhilemy K

更新日期：1990-10-01 00:00:00

abstract：:A coordinated up-regulation in the expression of all three neurofilament (NF) proteins occurs during postnatal development in the rat (Schlaepfer and Bruce, J Neurosci Res [in press], 1990a). In the present study, sciatic nerves were transected in neonatal rats in order to determine the effects of axotomy on the postn...

journal_title：Journal of neuroscience research

authors： Schwartz ML,Shneidman PS,Bruce J,Schlaepfer WW

更新日期：1990-10-01 00:00:00

abstract：:The neuroblastoma, N1E-115, was grown for 9 days after subcultivation. The development of acetylcholinestrase (AChE), choline acetyltransferase (CAT), QNB-binding, choline uptake, and acetylcholine release was measured on days 1, 3, 6, and 9. In parallel experiments the irreversible AChE inhibitor soman was added to n...

journal_title：Journal of neuroscience research

authors： Kumar U,Sellström A

更新日期：1990-09-01 00:00:00

abstract：:Butyrate produced a biphasic modulation of the thyroid hormone receptor in neuroblastoma N2A cells increasing receptor number by 20-35% at concentrations 0.25-0.75 mM and decreasing receptor levels by 30-55% at 2-4 mM. The half-life of the receptor, as assessed by its disappearance after incubation with 18 microM cycl...

journal_title：Journal of neuroscience research

authors： Yusta B,Ortiz-Caro J,Bedo G,Pascual A,Aranda A

更新日期：1990-09-01 00:00:00

abstract：:Previous histochemical studies of iron in the brain demonstrated iron enrichment in oligodendrocytes, but the iron-enriched oligodendrocytes had a spatially restricted distribution that excluded major white matter tracts. In this study we used techniques designed to permeabilize tissue, combined with iron histochemist...

journal_title：Journal of neuroscience research

authors： LeVine SM,Macklin WB

更新日期：1990-08-01 00:00:00

abstract：:The five major antioxidant enzymes, glutathione, and in vivo or in vitro stimulated (Fe(++)-ascorbate) peroxidation were similar in old and young Rana perezi frogs. Long-term (2.5 months) treatment with aminotriazole strongly decreased cerebral catalase (CAT) activity and increased in vivo but not in vitro peroxidatio...

journal_title：Journal of neuroscience research

authors： Barja de Quiroga G,Pérez-Campo R,López-Torres M

更新日期：1990-07-01 00:00:00

abstract：:The mast cell, an immunocompetent cell that contributes to neurogenic inflammation in a variety of tissues, including synovium, is found in close proximity to peripheral terminals of unmyelinated primary afferents and sympathetic postganglionic nerve terminals. In this study we evaluated the hypothesis that the densit...

journal_title：Journal of neuroscience research

authors： Levine JD,Coderre TJ,Covinsky K,Basbaum AI

更新日期：1990-07-01 00:00:00

abstract：:Isotonic media containing sodium salts from weak organic acids induce cell swelling in several experimental preparations (Grinstein et al., 1984; Jakubovicz et al., 1987). In vivo perfusion of rat dentate gyrus, using a microdialysis probe, with modified Krebs-Ringer bicarbonate solutions in which 50 mM NaCl was isoto...

journal_title：Journal of neuroscience research

authors： Solis JM,Herranz AS,Herreras O,Menéndez N,del Rio RM

更新日期：1990-06-01 00:00:00

abstract：:The role of axons in the expression of the major myelin glycoprotein, P0, has been investigated using neuron/Schwann cell cultures. These cultures were either nonmyelinating or myelinating due to growth in defined medium or in medium containing serum and chick embryo extract, respectively. The neurons and Schwann cell...

journal_title：Journal of neuroscience research

authors： Brunden KR,Windebank AJ,Poduslo JF

更新日期：1990-06-01 00:00:00

abstract：:Recent experiments have shown that Arg, Lys, and Leu can be incorporated posttranslationally into proteins of regenerating sciatic nerves of rats. The present experiments investigate a mixture of 15 radioactive amino acids to determine if additional amino acids can be conjugated posttranslationally to proteins of rege...

journal_title：Journal of neuroscience research

authors： Chakraborty G,Yu M,Luo D,Sturman JA,Ingoglia NA

更新日期：1990-04-01 00:00:00

abstract：:The distribution of both tyrosine hydroxylase (TH) antigen and messenger RNA was investigated in the ventral mesencephalon from human brain using a 35S-labeled human TH cDNA probe and a polyclonal TH antibody. A hybridization signal was detected not only in the perikaryon but also in the neurites, most probably dendri...

journal_title：Journal of neuroscience research

authors： Dumas S,Javoy-Agid F,Hirsch E,Agid Y,Mallet J

更新日期：1990-04-01 00:00:00